Agarose drop method for loading thin polyacrylamide gels

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Agarose drop method for loading thin polyacrylamide gels.

Thin gels (<I mm) can be loaded conveniently, rapidly, and quantitatively by suspending the sample to be analyzed in a drop of agarose gel and simply placing the solidified drop on top of the stacking gel. With this method there is no lower limit to the size of the sample to be loaded or to the thinness of the gel to be employed. Using the silver stain quantitation of between 1 and 1000 @ample ...

متن کامل

Agarose overlays allow simplified staining of polyacrylamide gels.

Laser scanners from Molecular Dynamics (Sunnyvale, CA, USA) or Hitachi Scientific Instruments (Mountain View, CA, USA) are being used increasingly to detect DNA in polyacrylamide gels. The standard technique for visualizing DNA is to separate the plates of a gel and pour a diluted nucleic acid stain such as ethidium bromide or SYBR Green I [from Molecular Probes (Eugene, OR, USA); also markete...

متن کامل

Improved method for enzymic determination of cholesterol in lipoproteins separated by electrophoresis on thin layer agarose gels.

The cholesterol of lipoproteins, separated electrophoretically on thin layer agarose films, is visualised and quantitated by incubating the gels in an enzymic reagent containing cholesterol esterase and cholesterol dehydrogenase. The individual fractions are quantitated by scanning densitometry. No sample pretreatment is necessary. All major fractions are detected readily. The accuracy of the d...

متن کامل

SDS agarose gels for analysis of proteins.

A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose, SeaKem Gold (FMC BioProducts). TBE containing sodium dodecyl sulfate (SDS) is used as electrophoresis buffer. The disadvantages of trad...

متن کامل

Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels.

Due to its avascular nature, articular cartilage exhibits a very limited capacity to regenerate and to repair. Although much of the tissue-engineered cartilage in existence has been successful in mimicking the morphological and biochemical appearance of hyaline cartilage, it is generally mechanically inferior to the natural tissue. In this study, we tested the hypothesis that the application of...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Analytical Biochemistry

سال: 1982

ISSN: 0003-2697

DOI: 10.1016/0003-2697(82)90538-3